"Fusaric acid alters Akt and ampk signalling in c57bl/6 mice brain tissue. It is designed to identify total protein changes and phosphorylation changes by comparing normal samples to treated/diseased samples. RayBio® C-Series Human/Mouse MAPK Phosphorylation Antibody Array 1 Kit. "?Journal of Biological Chemistry?295.20 (2020): 6946-6957. Glutamine, but not Branched-Chain Amino Acids, Restores Intestinal Barrier Function during Activity-Based Anorexia.?Nutrients. The Cell Signaling Phospho Antibody Array features 304 highly specific antibodies important to 16 commonly studied cell signaling pathways. For best results, incubate Part A and Part B in the same lysate preparation … All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/750 dilution Lane 1 : MCF-7 cell lysate with N-Peptide Lane 2 : MCF-7 cell lysate Lane 3 : MCF-7 cell lysate with P-Peptide Lane 4 : Mouse brain tissue lysate with N-Peptide Lane 5 : Mouse brain tissue lysate Lane 6 : Mouse … 17 Apr. Suitable for all liquid sample types but intended for use with cell and tissue lysates. Poling, Holly M., et al. These arrays are a great second step for large scale analysis such RNAseq. Detects 17 phosphorylated proteins in human or mouse samples. Web site www.bio-rad.com USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 … Furthermore, customers can choose to target all 3 types of phosphorylation (pSer/pThr/pTyr) by 2D DIGE PhosphoProtein Array, or a specific type by 2D Phospho … ErbBABL1, AKT1, AKT2, BAD, BRAF, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CBL, CDKN1A, CRK, EGFR, EIF4EBP1, ELK1, ERBB2, GAB1, GSK3B, MAP2K1, MAP2K4, MAPK9, MTOR, MYC, PAK1, PLCG1, PTK2, RAF1, RPS6KB1, SHC1, SRC, STAT5A, STAT5B , Focal AdhesionACTB, ACTG1, AKT1, AKT2, BAD, BCL2, BRAF, CCND1, CRK, CTNNB1, EGFR, ELK1, ERBB2, GSK3B, IGF1R, ITGB3, KDR, MAP2K1, MAKP9, MET, PAK1, PDGFRB, PDPK1, PPP1CA, PTEN, PTK2, RAF1, RASGRF1, SHC1, SRC, VASP. Visit our Frequently Asked Questions page. No problem! the surface of MSD Multi-Array 96-well plates coated with streptavidin Antibodies: Mouse anti-phospho-MBP IgG (primary) and MSD Sulfo-TAG™-labeled anti-mouse IgG (secondary) • Assay measures the activity of ERK kinase … They are easy to use, very clean membranes with super sharp results. This product is furnished for LABORATORY RESEARCH USE ONLY.Not for diagnostic or therapeutic use. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. Compare Kinase Multiplex Assays from leading suppliers on Biocompare. Higher density than ELISA, Western blot or bead-based multiplex, Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes, Pipettors, pipet tips and other common lab consumables, Tissue Paper, blotting paper or chromatography paper, A chemiluminescent blot documentation system (such as UVP? Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies. RTK Phosphorylation Array Semi-quantitative, Sandwich-based Array for Detecting RTK Phosphorylation Signatures The RayBio® Human RTK Phosphorylation Antibody Array is a rapid, convenient and sensitive assay for simultaneous detection of phosphorylation of 71 different human tyrosine kinase … The array is designed for protein phosphorylation profiling … 2018, doi:10.1155/2018/7413027. Detects 17 phosphorylated proteins in human or mouse samples. "Choice of factors and medium impinge on success of ESC to TSC conversion. Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1, p70 ribosomal S6 kinase alpha, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, KS6B1_HUMAN. Immunogen corresponding to synthetic peptide. Phospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. The Cell Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative profiling and screening of candidate biomarkers from 16 cell signaling pathways, including PI3K/AKT signaling, apoptosis, autophagy, cell cycle, ErbB, focal adhension, MAPK, p53 signaling, VEGF and more. RayBiotech also provides a GLP-compliant CRO testing service for this kit. Suitable for all liquid sample types but intended … Questions? R&D Systems' Mouse Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY014) is a screening tool designed to simultaneously detect the relative phosphorylation of 39 different RTKs. Article Title: Phospho-proteomic analysis of primary human colon epithelial cells during the early Trypanosoma cruzi infection phase Article Snippet: Antibodies and chemicals The proteome profiler human phospho-kinase array … ?s ChemiDoc-It, Incubate with Detection Antibody Cocktail, Image with chemiluminescent imaging system. Life Science Group Bulletin 2903 US/EG Rev F 07-0735 0907 Sig 1106 Bio-Rad Laboratories, Inc. Phospho-Kinase A protein kinase is a kinase enzyme that modifies other molecules, mostly proteins, by chemically adding phosphate groups to them (phosphorylation). Don’t have access to a compatible microarray scanner? BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis. Assays for analytes represented in the Mouse Phospho-Receptor Tyrosine Kinase Array Kit Receptor Tyrosine Kinases (RTKs) are widely expressed transmembrane proteins that act as receptors for … 2020 March 7. doi: 10.1016/j.fct.2020.111250, Ye Y, Wang H, Polygalasaponin F treats mice with pneumonia induced by influenza virus, Inflammopharmacology. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins … The Mouse Phospho-Receptor Tyrosine Kinase (Phospho-RTK) Array is a rapid, sensitive, and economical tool used to detect changes in phosphorylation between samples. Phosphorylation usually results in a … Phospho-array was used to screen kinase candidates related to expression change of CIP2A. Yu S., Zhong Q., Chen R., et al. Tel: 770-729-2992, 1-888-494-8555 (toll-free). "?Food and Chemical Toxicology? Dhani, Shanel, et al. UniProt … The ELISA based Cancer Signaling Phospho Antibody Array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin. Results are delivered by email in 1-3 weeks. Cited in 3 publication(s). All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Human Phospho-Kinase Antibody Array (Membrane, 43 Targets) (Y0225) (AbAr-0225-YC) Antibody array is a specific form of protein microarray. Assays for analytes represented in the Mouse Phospho-Receptor Tyrosine Kinase Array Kit … This service is free of charge. (2020): 111252. Rabbit polyclonal Fyn (phospho Y530) antibody. Simply send the finished array slides to our lab for scanning. 2018 Apr 17;2018:7413027. doi: 10.1155/2018/7413027. | MAPK8 | MTOR | RPS6KA1 | RPS6KA3 | RPS6KA4 | RPS6KB1 | TP53, RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA, RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA, RayBio Human/Mouse/Rat Phospho-GSK3a (S21) ELISA, RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA, RayBio Human/Mouse/Rat Phospho-MEK1 (S217/S221) ELISA, RayBio Human/Mouse/Rat Phospho-MKK3 (S189) ELISA, RayBio Human/Mouse/Rat Phospho-MKK6 (S207) ELISA, RayBio Human/Mouse Phospho-p38 alpha (T180/Y182) ELISA, RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA, RayBio Human/Mouse/Rat Phospho-P70S6K (T421/S424) ELISA, SIMOA - Single Molecule Protein Detection, Comprehensive Service and Discovery Center, Bulk Monoclonal Antibody Production Service, Application Notes & Technical White Papers. 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