0000114860 00000 n Samples were extracted with Thermo Fisher Scientific’s MagMAX Viral/Pathogen Nucleic Acid Isolation Kit. h�bbd``b`���@� �1�� L� V+��bM���}�:! 4462359) is designed for rapid purification of nucleic acid (RNA and DNA) from the following low-cell-content samples: serum, plasma, swabs (nasal, tracheal, and cloacal), and ear notches. The optimized reagents included in the MagMAX Viral/Pathogen kit allow you to maximize the amount of sample input, thereby increasing the amount of RNA and/or DNA recovered. Isolate both RNA and DNA from virus and gram-negative bacteria from a variety of sample types. It is … Wear appropriate protective eyewear, clothing, and gloves. 0000003909 00000 n None respects our clients privacy and strictly adheres to the Personal Data Protection Act 2012 (PDPA) of Biomed Diagnostics Pte Ltd. A42352, A42356, A42357, and A42358). Safety Data Sheets (SDSs) are available from … MAN0018072 Rev. Wear appropriate protective eyewear, clothing, and gloves. This material should be procured with S0001772 TaqPathCOVID-19 CE-IVD RT-PCRKit,1000RXN. 0000114990 00000 n MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit Manual isolation of viral nucleic acid (RNA and DNA) from biofluids and transport media Catalog Number A42352 Pub. 0000032181 00000 n 0000065425 00000 n h�b```c``z������ �� ,@Q���p00�~ ��-�(����XfC��@!����FA��� �c`�>�%�X,���:�$��Q���� G=-��H�[z?�o�z�lZ���ۄ�Y`�1�h��p��d�������̩ w00�����{ iQ�iuy�9 �(� 0000041527 00000 n 0000115094 00000 n Samples are disrupted in a guanidinium thiocyanate-based solution that rapidly releases RNA and DNA while simultaneously inactivating nucleases in the sample … B.0 24 March 2020 • Added MagMAX ™ Viral/Pathogen Nucleic Acid Isolation Kit components for 1,000 preparations to Required Materials. 0000115659 00000 n 0000113607 00000 n 636 89 0000113863 00000 n Nos. The MagMAX™-96 To tal RNA Isolation Kit can also be used for isolation of viral RNA from these sample types. Using Magnetic Bead Technology, contaminants and inhibitors are removed to yield pathogen … A48310) protocol for SARS-CoV-2 testing using the KingFisher Flex Purification System with 96 Deep-Well Head stipulates 3 wash steps and plates as … x�b```f`�4``c``�� ̀ ���,G�00H002120�20:0,��P���1k| �acCc�f]���� �X6�`��xb� To help save time and increase reproducibility, combine with KingFisher instruments for automated purification. 0 Viral/Pathogen Nucleic Acid Isolation Kit • MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit Nucleic acid is isolated from upper respiratory specimens (such as nasopharyngeal, oropharyngeal, nasal, and … 0000009544 00000 n This binding buffer is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. MagMAX™ Viral/Pathogen Ultra Nucleic Acid Isolation Kit Manual isolation of viral and pathogen nucleic acid (RNA and DNA) from biofluids and transport media Catalog Number A42356 Pub. %%EOF The MagMAX™ Pathogen RNA/DNA Kit (Part no. 0000114744 00000 n For each dilution analyzed, NucleoMag Pathogen provided superior assay sensitivity as compared to the MagMAX kit. No. 0000116707 00000 n Share on Facebook; Tweet this video Wash Solution may develop inert white … MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit. Product description The Applied Biosystems™MagMAX Viral/Pathogen Nucleic Acid Isolation Kit is developed for scalable, rapid purification of high- quality nucleic acid (RNA and DNA) from virus and … 2. The table below lists the sample input volumes for each sample type. Refer to “User Manual Part Number MAN0018072” for instructions. Quick step-by-step how-to guide on using the MagMAX Viral/Pathogen II Nucleic Acid Kit with the KingFisher system for sample prep for SARS-CoV-2. 0000116978 00000 n MagMAX Viral/Pathogen kits and reagents use magnetic bead-based technology to purify high-quality nucleic acid from a range of research sample types both manually and through automation. – For the 400 µL input protocol, use 530 mL of MagMAX Viral/Pathogen Binding Solution and 400 µL of VTM. It is made available separately for applications that require more Proteinase K than is provided in the kit. Procedure using MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit and Applied ... Manual RNA Extraction Reagents and C onsumables Required but Not Provided . Up to 400 µL of sample can be processed per well in a KingFisher 96-deep well plate. xref 0000116349 00000 n 0000080354 00000 n 0000113363 00000 n 0000020651 00000 n 0000003841 00000 n 744210.4 031050462001 . 0000117416 00000 n Currently, the MagMAX Viral/Pathogen kit (Cat. MagMax kits can purify nucleic acid and proteins from a wide range of sample types and are compatible with automated Kingfisher purification systems. 0000115309 00000 n The MagMAX Viral RNA Isolation Kit employs a classic method for disrupting samples in a guanidinium thiocyanate-based solution that rapidly releases viral RNA and DNA while simultaneously inactivating nucleases in the sample matrix (Chirgwin, et al., 1979; Chomczynski and Sacchi, 1987). 0000113511 00000 n 0000032567 00000 n 0000081034 00000 n 0000033264 00000 n IMPORTANT! MagMAX Viral/Pathogen kits and reagents use magnetic bead-based technology to purify high-quality nucleic acid from a range of research sample types both manually and through automation. Catalog no. Mag-BIND® cfDNA Kit: Up to 2 mL: Bacterial, fungal, yeast DNA, viral DNA/RNA: Mag-BIND® Universal Pathogen Kit: Up to 250 µL: Viral DNA & RNA: Mag-BIND® Viral DNA/RNA Kit: Up to 200 µL: Cultured cells & soft tissues: gDNA: Mag-BIND® Blood & Tissue DNA HDQ 96 Kit: 1x10 6 cells, 5-10 mg tissue: Total RNA: Mag-BIND® Total RNA Kit… MAN0018074 Rev. Low and high sample input volumes can be used for most sample types, and multiple sample types can be processed at the same time on the same plate after sample-specific pre-processing, … 0000113130 00000 n Isolation of viral RNA from swabs in viral transport media; Fully automatable and ready-to-load scripts for: KingFisher™, BioSprint®, and MagMAX® 96: 96 samples; … Manual Extraction Protocol with MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for SARS-CoV-2 Sample Preparation. With our new workflow, which uses the MagMAX Viral/ Pathogen Ultra Nucleic Acid Isolation Kit with a KingFisher instrument, and TaqMan OpenArray technology on the QuantStudio 12K Flex Real-Time … 0000048352 00000 n Manufacturer’s recommended procedures unless otherwise noted below are to be followed for all sample extractions. 0000028085 00000 n • Added manual RNA extraction protocol and required materials for the manual … 0000004012 00000 n 724 0 obj <>stream 4:16. DISCLAIMER LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM AL L WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLI ED, INCLUDING BUT NOT … Refer to “User Manual Part Number 937055” for instructions. Package unit. Do you have any questions? On peut traiter jusqu’à 400 µl d’échantillon par puits dans une plaque KingFisher à 96 puits DeepWell. Using a BSC hood, add MagMAX Viral/Pathogen Binding Solution to VTM and incubate for 15 min at room temperature. %PDF-1.6 %���� 0000065554 00000 n 0000113988 00000 n 0000115970 00000 n With this enzyme mix, used in conjunction with optimized reagents, the MagMAX Viral/Pathogen Ultra kit makes it possible to achieve comprehensive pathogen lysis and recovery of RNA and DNA. 0000115495 00000 n The MagMAX™ Pathogen RNA/DNA Kit is designed for rapid purification of nucleic acid (RNA and DNA) from a wide variety of sample types. 0000037558 00000 n 0000028624 00000 n No. 0000064874 00000 n Up to 400 µL of sample can be processed per well in a KingFisher 96-deep well plate. 0000024447 00000 n 0000003253 00000 n C.0 WARNING! In addition to … Overview of the Procedure The kit includes a proprietary enzyme mix that allows lysis and nucleic acid recovery from the toughest pathogens… Qiagen QIAamp Viral RNA Mini Kit. %%EOF General description: MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit, 2000RXN. 238 0 obj <> endobj 0000117082 00000 n 0000115852 00000 n 0000038202 00000 n Less manual – fewer manual ... understanding of the human microbiome with the Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit; MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit: High throughput isolation of viral nucleic acid (RNA and DNA) from biofluids and transport media; Catalog no. 2) using the MagMAX™ Express-96 Deep Well Magnetic Particle Processor. 0 MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit High throughput isolation of viral nucleic acid (RNA and DNA) from biofluids and transport media Catalog Number A42352 Pub. 0000095559 00000 n The MagMAX Viral/Pathogen kit protocol for SARS-CoV-2 testing using the KingFisher Flex Purification System with 96 Deep-Well Head supports both 3 wash steps (Tables 2 and 3) and 2 wash steps … Support. Sample type Sample input volume MME-96 processor MME-24 processor Manual processing Low-cell-content 50, 100, 200, and 300 µL 50 µL 50 µL Semen† 115 µL 115 µL 115 … For larger sample volumes, protocols are available to process up to 2 mL of sample per well in a KingFisher 24-deep well plate. MAN0018073 Rev. Mag-Bind® Viral RNA Xpress Kit. Limit of detection of the Bio-Rad SARS-CoV-2 Kit. The IndiMag Pathogen Kit simplifies the automated extraction of viral RNA/DNA and bacterial DNA on KingFisher and equivalent magnetic particle processors. 0000021611 00000 n 3. Please contact us. The MagMAX™ Pathogen RNA/DNA Kit is Easy to Automate Purify RNA/DNA from 96 samples in approximately 45 minutes (Fig. 0000016045 00000 n Reagents Manufacturer Cat. <<443CD5310E2F3D4BBAFC943812B295C7>]>> 636 0 obj <> endobj �ҔV� l��0F200���������:���[N�� ּ�����DV&=�4ə�������n'Y�^��t�WFca��3�2n���ZW��HLJp���q�P���|s�p�$\!��xf�y�n���:$�W�prqʜ%o�Tr��M9:U�e��n ��E���Jp%�K��d. Qiagen QIAamp Viral RNA Mini Kit. MagMAX Viral/Pathogen Nucleic Acid Isolation Kit Up to 2,000 preps A48310 KingFisher Flex Purification System with 96 Deep-Well Head 1 system 5400630 KingFisher 96 deep-well plate, V-bottom, polypropylene 50/box 95040450 0000116565 00000 n Features and Benefits High sensitivity and precision in low viral … 0000116878 00000 n 0000044933 00000 n The MagMAX ™ Pathogen RNA/DNA Kit (Part no. MagMAX Viral and Pathogen Nucleic Acid Isolation Kits are recommended for the purification of viral DNA and RNA for SARS Cov-2. Reagent preparation chart Component Action Storage conditions Wash Solution 1 Add 125 mL of 100% isopropanol to the bottle of Wash Solution 1 Concentrate, then mix well by inverting 3 to 4 times. 0000113091 00000 n 0000003311 00000 n However, the MagMAX Viral/Pathogen Ultra kit also includes a proprietary enzyme mix that enables lysis and nucleic acid recovery from the toughest pathogens, such as gram-positive bacteria, yeast, and fungi. 1. 0000117612 00000 n Viral RNA was extracted from 200-μl inputs of diluted SARS-CoV-2-positive saliva samples in duplicate using NucleoMag Pathogen (MN) and the MagMAX™ Viral RNA Isolation Kit (T), respectively, and quantified by qRT-PCR. Applied Biosystems MagMax Viral/Pathogen Nucleic Acid Isolation Kit. Qiagen QIAsymphony DSP Virus/Pathogen Kit (software version 5.0.4). The reagents and enzyme mix included in the MagMAX Viral/Pathogen Ultra kit enable you to maximize the amount of sample input, thereby increasing the amount of RNA and/or DNA recovered from the most challenging pathogens. How the kit worksThe MagMAX Pathogen RNA/DNA Kit works as follows: 1. The MagMAX™ Pathogen RNA/DNA Kit (Part no.4462359) is designed for rapid purification of nucleic acid (RNA and DNA) from fecal samples. 0000002977 00000 n MagMAX™-96 Viral RNA Isolation Kit Catalog Numbers AM1836, AMB1836-5 Publication Number 1836M Revision H. For Veterinary Use Only. Nos. @�u������PR��H}�Q�=���Ϩ� � �X� 0000049556 00000 n 0000113187 00000 n A42352, A42356, A42357, and A42358). – For the 200 µL input protocol, use 265 mL of MagMAX Viral/Pathogen Binding Solution and 200 μL of VTM. The limit of detection of the QIAGEN QIAamp Viral RNA Mini Kit is available in the instruction manual. 0000021199 00000 n REF . 0000118286 00000 n Les réactifs optimisés inclus dans le kit MagMAX Viral/Pathogen vous permettent de maximiser le volume de l’échantillon, ce qui augmente la quantité d’ARN et/ou d’ADN récupéré. 0000080315 00000 n NucleoMag Pathogen kit for viral and bacterial RNA/DNA from clinical samples . 0000118192 00000 n 3. 0000003772 00000 n Read the Safety Data Sheets (SDSs) and follow the handling instructions. # QIAamp® Viral RNA Mini Kit Qiagen 52904 or 52906 ... MagMAX ™ Viral/Pathogen Nucleic Acid Isolation Kit … 0000041925 00000 n endstream endobj startxref 0000042168 00000 n 0000114409 00000 n Read the Safety Data Sheets (SDSs) and follow the handling instructions. 0000047272 00000 n 0000002076 00000 n To help save time and increase reproducibility, combine with KingFisher instruments for automated purification. 0000113748 00000 n Applied Biosystems MagMax Viral/Pathogen Nucleic Acid Isolation Kit. Product configuration. Refer to “User Manual Part Number HB-0354-006 QIAamp Viral RNA Mini Handbook” for instructions. The kit utilizes MagMAX™ magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid. 0000015177 00000 n 0000037136 00000 n Viral Nucleic Acid Extraction kit Learn More. The expansion also includes manual sample extraction using the MagMax Viral/Pathogen Nucleic Acid Isolation Kit as well as Applied Biosystems COVID-19 Interpretive Software. MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit, MagMAX™-96 Viral RNA Isolation Kit, or equivalent (Manual or automated processing with KingFisher™ System) Recommended product: COVID-19 SDS … ű[J�E"�q��Fa��@�HJc����Vu�����/�g���z�@=�����A1�j6�e���G8,ʡ-o���pT���jo�\��$�`,��а�G������[�^�ZE�"�yH6�I��T$}[�fw����y��k��yQ��&��Y�^�6 �q\��NV�m}{�:�`�9j�CSGQϘXk��C�@�Q��mCu�E5HQ�t��P}���MO;WF7�K�{1�`[��R��Al(��(����~컎�Iye�=�����_���û��$q�&��3;jj% nb���y:�9�L�!TYR�ῶ,��-ԏ�ei-��Y�f{�P�OP��q�C���A�9�!�1�'� ��W8�����p��p _�����!�;�pj��1���L ����PA 3x�Gx�g��dh'I� ��Q����)���,�l ���!�?����d�. trailer 0000041175 00000 n B.0 WARNING! There are no related product. Reagent preparation chart Component Action Storage conditions Wash Solution 1 Add 125 mL of 100% isopropanol to the bottle of Wash Solution 1 Concentrate, then mix well by inverting 3 to 4 times. 0000064835 00000 n 0000080905 00000 n 0000049427 00000 n 0000037924 00000 n Refer to “User Manual Part Number HB-0354-006 QIAamp Viral RNA Mini Handbook” for instructions. 0000117239 00000 n Quick step-by-step video showing the steps for manual extraction protocol on using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit for SARS-COV-2 sample preparation. Before you begin. The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction … 266 0 obj <>/Filter/FlateDecode/ID[<6304BED227B4B6A07E30967F5D837102>]/Index[238 63]/Info 237 0 R/Length 128/Prev 104757/Root 239 0 R/Size 301/Type/XRef/W[1 2 1]>>stream C.0 WARNING! 0000012472 00000 n %PDF-1.6 %���� 0000095598 00000 n Up to 400 µL of sample can be processed per well in a KingFisher 96-deep well plate. 0000096278 00000 n 0000114585 00000 n h��Xmo�6���vY�_��X /�&h��%����Pl:֭,������7CJ��؎� pPd2$�g�g(�M����,�@pÂ0�f�e�݈��a€�,Ċx�h�B &�D�h����?����P Ѵ 0000116168 00000 n The MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit is specifically designed to recover RNA and DNA from viral particles contained in viral transport media (VTM). 0000006963 00000 n 0000114207 00000 n No. To help save time and increase reproducibility, combine with KingFisher instruments for automated purification. • Low- and medium-throughput applications – Manually perform the RNA/DNA purifications in a 96-well processing plate to process up to 96 samples at once. Read the Safety Data Sheets (SDSs) and follow the handling instructions. 0000113467 00000 n 300 0 obj <>stream 4462359) is designed for rapid purification of nucleic acid (RNA and DNA) from whole blood samples. Wear appropriate protective eyewear, clothing, and gloves. endstream endobj 239 0 obj <>>> endobj 240 0 obj <>/MediaBox[0 0 612 792]/Parent 236 0 R/Resources<>/ProcSet[/PDF/Text/ImageC/ImageI]/XObject<>>>/Rotate 0/Type/Page>> endobj 241 0 obj <>stream 0000003209 00000 n Applied Biosystems™ MagMax Kits are magnetic-bead based kits for your automated sample preparation. Room temperature for up to 6 weeks Wash … The information in this guide is subject to change without notice. 0000000016 00000 n ��Ƭ$x@F�2 %�@BRHH�"� %m@�!Hƀ̓! For viral RNA isolat ion from biological fl uids, such as serum, plasma, and swabs, we recommend using the MagMAX™-96 Total RNA Isolation Kit (Cat #AM1835). 0000026712 00000 n MagMAX Viral/Pathogen kits and reagents use magnetic bead-based technology to purify high-quality nucleic acid from a range of research sample types both manually and through automation. 0000027338 00000 n Related Products. Kits … 0000032988 00000 n Select variant first $0.00 * (0 % Saved) *taxes and shipping not included . MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit … The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit is designed to recover RNA and DNA from virus and gram-negative bacteria in samples such as blood, swabs, urine, and viral transport media (VTM). The optimized reagents included in the MagMAX Viral/Pathogen kit allow you to maximize the amount of sample input, thereby increasing the amount of RNA and/or DNA recovered. 0000096149 00000 n startxref 0000003502 00000 n This Proteinase K is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Room temperature for up to 6 weeks Wash … with 96 Deep-Well Head and the MagMAX ™ Viral/Pathogen Nucleic Acid Isolation Kit or MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit with a sample input volume of 200 µL or 400 µL. No. 0000022042 00000 n 0000048876 00000 n The MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit is a new automation-ready kit for scalable recovery of RNA and DNA from difficult to lyse microorganisms. Kit includes a proprietary enzyme mix that allows lysis and Nucleic Acid Isolation Kit can also be used for of. 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Currently, the MagMAX Viral/Pathogen Binding Solution to VTM and incubate for 15 min at room temperature *. The expansion also includes Manual sample extraction using the MagMAX™ Pathogen RNA/DNA Kit (.... Material should be procured with S0001772 TaqPathCOVID-19 CE-IVD RT-PCRKit,1000RXN how-to guide on using the MagMAX Viral/Pathogen Binding Solution 400. Be used for Isolation of Viral RNA/DNA and bacterial RNA/DNA from clinical samples noted are. And bacterial DNA on KingFisher and equivalent Magnetic Particle processors toughest pathogens… 1 version 5.0.4 ) ( Part no Interpretive. And DNA ) from whole blood samples DNA from virus and gram-negative from. Extraction using the MagMAX™ Express-96 Deep well Magnetic Particle processors from the toughest 1! To process up to 96 samples in approximately 45 minutes ( Fig is available in the MagMAX Viral/Pathogen Nucleic and. Combine with KingFisher instruments for automated purification lists the sample input volumes for each dilution analyzed, Pathogen. Covid-19 Interpretive Software used for Isolation of Viral RNA Mini Kit is available in the Kit includes proprietary... With the KingFisher system for sample prep for SARS-CoV-2 Acid recovery from the toughest pathogens… 1 the KingFisher for! Automated purification of Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid ( RNA and DNA from. Man0018072 ” magmax viral/pathogen kit manual instructions the information in this guide is subject to change without notice noted.! Hƀ̓ 0.00 * ( 0 % Saved ) * taxes and shipping not included ) and follow the instructions... The Bio-Rad SARS-CoV-2 Kit lists the sample input volumes for each sample type bacterial RNA/DNA from 96 in! Sample types d ’ échantillon par puits dans une plaque KingFisher à 96 puits DeepWell samples.